![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4458005:Design
Designed by: Pavin Rajagopal Group: iGEM22_East_Coast_BioCrew (2022-09-10)
pcbA4 assembly
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1
Illegal PstI site found at 131
Illegal NotI site found at 19 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 13
Illegal XbaI site found at 234
Illegal PstI site found at 1
Illegal PstI site found at 131
Illegal AgeI site found at 1430 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1393
Design Notes
Codon optimization for rhodococcus jostii RHA1 , full part gene assembly
Source
Dehalococcoides mccartyi assembly